Candida has been reported has one of the most prevailing yeast genera. Candida is of diverse species that are commonly leave on soil and in the mucosal surfaces of genitourinary tract, gastrointestinal tract, and the mouth of human and have ability to cause oral thrush or vaginal thrush. The incidence of Candida infections has dramatically increased in recent years due to a significant increase in cases like HIV/AIDS, cancer, diabetes mellitus, long time use of antibiotic and tissue transplant. It is a cross-sectional study involving patients diagnosed of Candidiasis. The aim of this study is to identify, characterize and determine the prevalence of Candida species isolated from clinical specimens in selected health care facilities in Ilorin, Kwara state, Nigeria using conventional methods such as CHROM agar Candida differential media and Germ tube test. Of the 232 isolates recovered from Sabouraud dextrose agar, sub-cultured on CHROM agar and tested for Germ Tube Test. C. albicans 131(56.5%), has the highest prevalence, non-albicans such as C. tropicalis has the prevalence of 36(15.5%), C. dubliniensis 27(11.6%), C. glabrata 14(6.0%), C. krusei 12(5.2%), there was mixed growth 7(3.0%) and 3(1.3%) did not grow on CHROM agar. Candida albicans is the most prevalence of Candida species isolate from clinical specimen in Ilorin, Nigeria in this study (56.5%) and C. albicans is also the most prevalence in Female (59.6%) while C. tropicalis has the highest prevalence in Male (36.8). Age group between 20 -29 has the highest prevalence (64.5%). Clinical specimens that produce highest number of Candida species in this study was High Vaginal Swab (58.2%) and pelvic inflammatory disease was the most clinical diagnosis (34.1%) and female subject has the highest prevalence of Candida infections (91.8%) while male has (8.2%) in Ilorin, Nigeria. There was a statistical significant difference (P < 0.01) between age and Candida species distribution among participant.