The purpose of this study is to find out the impact of passive and active teaching methods on students’ learning in Yenagoa, Bayelsa State. Pretest-posttest experimental design was adopted for this study. Two hundred secondary school students were randomly selected for this study (X = 14.42; SD = 0.91). The sample was made of 72 males and 128 females. Data was collected via administration of achievement test in Biology with specific focus on Osmosis and Diffusion. Frequency count, percentages, ANOVA and MANOVA were used to analyse data. The findings showed that the traditional lecture method (verbal teaching) was the most commonly used teaching method in public secondary schools in Yenagoa, Bayelsa State. Out of the four teaching methods assessed, students attain 61% level of learning with video watching method, 70% with traditional lecture method, 84% with practical teaching method and 85% with discussion method. On the overall, active teaching methods had significant impact on students’ learning than the passive teaching method at p<.05 level for the four conditions (F (3,192) = 162.03, p = .000). However, gender factor appeared not to have significant effect on students’ learning. Based on these finding, it was recommended that the Ministry of Education should embark on enlightenment campaign on the need for teachers to employ effective teaching methods in classroom.Â