Mortalities due to pathogenic bacteria are a major problem in aquaculture. The use of probiotics is now a better option than antibiotics in disease control. Investigations on Pseudomonas aeruginosa strain XyC5 isolated from healthy shrimp intestine and found to protect Penaeus monodon from Vibrio mimicus infection were carried out. The isolate was screened for qualitative enzyme activity, antibiotic susceptibility and environmental stress tolerance. Assessment of its bioactive compounds and effects of abiotic factors on growth were also studied. Capability for enzyme production was investigated via starch agar, skim milk agar, egg yolk agar, cellulose agar and gelatin agar for amylase, protease, lipase, cellulase and gelatinase activities respectively. The effects of abiotic factors on growth of the isolate were carried out by cultivating the isolate in nutrient broth at different temperature, salinity and pH levels. GC-MS was employed for analysis of methanol extracted metabolites. The isolate produced hydrolytic enzymes such as amylase, protease, gelatinase and lipase. The vulnerability of the isolate to all antibiotics tested ensures its incapability to transfer antibiotic resistance. The isolate exhibited a broad range of ecological tolerance with temperature (28-40oC), salinity (0-100 ppt) and pH (4-11). The optimum temperature, salinity and pH were 37oC, 10-20 ppt. and pH 6-8 respectively. Fourteen compounds were produced with Tromethamine (C7H11NO3) as the major compound. These results imply that Pseudomonas aeruginosa strain XyC5 is a prospective candidate organism for drug improvement for aquaculture systems.