In this study, the prevalence of bacterial and fungi species on toilet door handles in male and female hostels and their antibiotic susceptibility patterns were assessed. A total of 48 swab samples were collected, and 21 isolates were identified through cultural, morphological, and biochemical and comparison with standard organisms. Fifty seven percent isolates (57%) were found on female toilet door handles and 43% on the male hostels. The identified isolates included Citrobacter freudii, Enterobacter spp, Bacillus subtilis, Klebsiella pneumoniae, Bacillus megaterium, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida spp. Antibiotic susceptibility patterns revealed that all isolates were resistant to cefuroxime, cefotaxime, and vancomycin (100% resistance). However, the organisms exhibited high susceptibility to Amikacin (100%), Ciprofloxacin (89%), and Cephalexin (86%).