Standard precautions (SP) protect people against potential transmission and outbreak of communicable disease, and complying with SP, medical professionals and patients attain good health and well-being. The study was conducted to evaluate the knowledge, attitude and practice of SP among two hundred and thirty two (232) healthcare workers in Kogi State Specialist Hospital (KSSH). The healthcare workers were selected through a multistage sampling technique, and the instrument used was self-administered questionnaire. Result from the findings showed that all KSSH healthcare workers had heard about SP among which 84.5% majority of them heard about SP through seminars/workshop/training. More result from the findings showed that 60.8% majority
of KSSH healthcare workers had poor knowledge on SP, 92.2% majority of KSSH healthcare workers had positive attitude towards SP, and 96.6% majority of them practice good SP. The factors that significantly influences the practice of SP are female healthcare workers [AOR = 2.027; CI = 1.534 – 2.678], healthcare workers between the ages of 30 – 39 years [AOR = 4.550; CI = 2.804 – 7.383], married healthcare workers [AOR = 15.000; CI = 8.556 – 26.298], Christians [AOR = 3.073; CI = 2.267 – 4.165], KSSH hospital attendant
[AOR = 4.684; CI = 2.854 – 7.678], KSSH healthcare workers with good knowledge [AOR = 1.545; CI = 1.182 – 2.021], and healthcare workers with positive attitude [AOR = 12.176; CI = 7.426 – 19.965]. This study showed that KSSH healthcare workers have poor SP knowledge, although they exhibited positive attitude, and good practiced of standard precaution.