The aim of this study was to determine the prevalence of Hepatitis B virus infection among inmates of selected correctional facilities in Niger state, Nigeria. Blood samples were collected from inmates in three correctional facilities (Bida, Kontagora and Minna). Questionnaires were administered to get their bio-data and 5ml of blood sample was collected from a total of 344 inmates. The plasma was separated and tested for hepatitis B surface antigen (HBsAg) using rapid chromatographic immunoassay test (ICT) and enzyme linked immunosorbent assay test kits (ELISA) . All the HBsAg positive samples were subjected to further test using 5-panel HBV test card. Out of the 344 samples collected, 75 (22%) were positive by ICT for HBsAg. ELISA gave an overall prevalence rate of 25% (87/344) as additional 12 samples were positive. The result of the 5-panel showed that HBsAg, HBsAb, HBeAg, HBeAb and HBcAb were present in 87, 19, 20, 47 and 68 plasma respectively. This implies that 87 persons were infected, 19 had immunity against the virus, 20 had active viral replication, 47 with no viral replication and 68 with onset of acute infection. The HBV infection was highest in the age bracket 21-30 years (29.7%) and lowest in 61-70% (0%). Out of the associated risk factors, sharing of objects showed statistically significant association with the high prevalence of the HBV. This study showed the prevalence of HBV among inmates. As such, there is need for constant screening of the inmates for effective prevention measure and proper clinical management strategy.