Improper crop and animal waste management and disposal are now widely recognized as environmentally harmful practices. When crop waste is placed in landfills, greenhouse gases (GHG) like carbon dioxide (CO2) and methane (CH4) are created. In order to create the digestate for usage as bio-fertilizer from agricultural waste employing cow dung as an inoculum for 30 days of the hydraulic retention period, a 225L Polyethylene (PE) anaerobic digester was used in this study. Cassava peels, palm oil sludge, cow dung, and water were mixed in a 1:1:2:5.3 ratio. About 1.3 kilogram of crushed eggshells was added to keep the pH level within the range recommended for the anaerobic digestion process. In order to maintain mesophilic conditions during anaerobic co-digestion for enhanced organic fertilizer output, the greenhouse was used to regulate temperature. Since microbial populations flourish in friendly environments, the pH averaged 6.0 and the average slurry temperature was 34.76 oC during digestion. After 30
days of hydraulic retention time, a laboratory-scale elemental analysis of the digestate showed that the contents of nitrogen (N), phosphorus (P), and potassium (K) increased by 95%, 75%, and 93.8%, respectively. The anaerobic co-digestion of animal and agricultural waste has created digestate rich in NPK nutrients, but more research should be conducted to see whether the biofertilizer’s efficacy on fast-growing crops can be determined by measuring the number of harvests and height of the plants.