It is imperative to develop a means of utilizing municipal solid waste incinerator ash (MSWIA) for a proper environmental protection. Hence, this research is to evaluate the influence of municipal solid waste incinerator ash (MSWIA) as a substitute for fine aggregate in hot-mix asphalt concrete. The physical properties and chemical oxide composition tests were carried on MSWI ash with the view of evaluating its suitability for used as fine aggregate. Marshall mix design method was adopted for sample preparation. Preliminary samples were prepared to determine the optimum bitumen content (OBC). The OBC was determined as 5.5% and was used for the preparation of samples with varying proportion of MSWIA (10%, 20%, 30% and 40 by total weight of the fine aggregate). The samples were subjected to mechanical and volumetric evaluations, which include stability, flow, bulk specific gravity, void in mineral Aggregate (VMA) void filled with bitumen (VFB) and Voids in the mix (VIM). The results revealed that the flow, void in mix (VIM), void in mineral aggregate (VMA) increased, while bulk specific density (BSD) and void filled with bitumen (VFB) became less as the percentage of MSWI ash raised. However, the use MSWI ash in the preparation of hot mix asphalt as a substitute for fine aggregate should not be beyond 20 % MSWIA. Moreover, the results fulfilled the Standard Specification and requirements specified by Nigerian Standard Specification for Roads and Bridges.